mir sensor assay  (Addgene inc)

Journal: ACS Omega

Article Title: miR29a-Loaded Extracellular Vesicles Derived from Human Mesenchymal Stem Cells Inhibit Fibrotic and Inflammatory Signaling

doi: 10.1021/acsomega.5c03490

Figure Lengend Snippet: Representative images of DAPI (blue), F-Actin (magenta), and YAP (white) stained MSCs post transfection with miRNA mimics for miR29a or a scrambled mimic (A). Nontransfected MSC group used as control and the nuclear to cytoplasmic ratio of YAP and the cell area was quantified (B, C). (Violin plots indicate mean, quartiles and distribution, n = 132–163 cells/grp). One-way ANOVA with Dunnett’s post hoc ** p < 0.01, ** p < 0.001. Scale for all images = 50 μm.

Article Snippet: A previously described miR29a miRNA sensor (LSB-hsa-miR-29a-3p, Addgene 103391) was transiently transfected into 3T3 fibroblasts and used for miRNA sensor experiments to test delivery and targeting.

Techniques: Staining, Transfection, Control

Journal: ACS Omega

Article Title: miR29a-Loaded Extracellular Vesicles Derived from Human Mesenchymal Stem Cells Inhibit Fibrotic and Inflammatory Signaling

doi: 10.1021/acsomega.5c03490

Figure Lengend Snippet: Diagram showing the miRNA sensor construct. With the addition of miR29 and targeting of the miRNA response element (MRE), expression of mKate is reduced (A). Representative images of LSB-has-miR-29a-3p sensor-transfected NIH-3T3 fibroblasts stained with 650 Nuclear Dye after 24 h treatment with Neg. MSC EVs or miR29a mimic-transfected MSC EVs (B). The mKate to BFP signal intensity ratio was quantified for cells treated with MSC EVs (C). (mean ± SEM, n = 106–123 cells/grp). One-way ANOVA with Dunnett’s post hoc * p < 0.05. Scale for all images = 50 μm.

Article Snippet: A previously described miR29a miRNA sensor (LSB-hsa-miR-29a-3p, Addgene 103391) was transiently transfected into 3T3 fibroblasts and used for miRNA sensor experiments to test delivery and targeting.

Techniques: Construct, Expressing, Transfection, Staining

Journal: ACS Omega

Article Title: miR29a-Loaded Extracellular Vesicles Derived from Human Mesenchymal Stem Cells Inhibit Fibrotic and Inflammatory Signaling

doi: 10.1021/acsomega.5c03490

Figure Lengend Snippet: HDF-seeded collagen gels after 1 week of contraction with treatment of Scr. or miR29a EVs at 1e8 EV/gel (A). (scale bar = 5 mm). Quantification of area of gel normalized to the area of well and the no EV group (B). (mean ± SEM, n = 7 gels/grp). Fold change in CTGF expression of collagen gels (C). (mean ± SEM, n = 3/grp). Representative images of HDF-seeded collagen gels stained with Hoechst (blue), F-Actin (green), and YAP (white) (D). (scale bar = 50 μm). YAP quantification of cells seeded within collagen gels after contraction (E). (mean ± SEM, One-way ANOVA with Dunnett’s post hoc, * p <0.05, *** p <0.001).

Article Snippet: A previously described miR29a miRNA sensor (LSB-hsa-miR-29a-3p, Addgene 103391) was transiently transfected into 3T3 fibroblasts and used for miRNA sensor experiments to test delivery and targeting.

Techniques: Expressing, Staining